The Liverpool legend sung a rap for England's football World Cup campaign in 1990. The original World In Motion video showed Barnes on a training pitch, miming rap lyrics with a football tucked under his arm.
The new advertising campaign, of about 30 seconds, will be filmed next week and launched in May on television and online. The track is meant to encourage fans to sing along in support of England's football team.
The chocolate brand, Mars, will offer the chance to 15 people to appear alongside Barnes in the new advertisement.